3LYW

Crystal structure of YbbR family protein Dhaf_0833 from Desulfitobacterium hafniense DCB-2. Northeast Structural Genomics Consortium target id DhR29B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-24
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	44.827, 44.827, 85.997
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.520 - 1.900
R-factor	0.224
Rwork	0.224
R-free	0.25800
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.058	0.412
Number of reflections	15491
<I/σ(I)>	14.5	24
Completeness [%]	99.6	100
Redundancy	17.7	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	30% PEG3350, sodium acetate 10mM, 5% Glycerol, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K