3LYV
Crystal structure of a domain of ribosome-associated factor Y from streptococcus pyogenes serotype M6. Northeast Structural Genomics Consortium target id DR64A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.602, 64.677, 69.563 |
| Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
| Resolution | 47.220 - 2.700 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.28400 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.067 | 0.361 |
| Number of reflections | 27508 | |
| <I/σ(I)> | 25.3 | 24 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.14M (NH4)2SO4, 0.1M MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
