3LYP

Structure of stringent starvation protein A homolog from Pseudomonas fluorescens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	43.591, 53.917, 54.496
Unit cell angles	85.28, 71.05, 66.71

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.185
Rwork	0.183
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yy7
RMSD bond length	0.013
RMSD bond angle	1.528
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.073	0.071	0.201
Number of reflections	54196
<I/σ(I)>	17.7
Completeness [%]	95.4	86.5	94
Redundancy	3.2	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.1 M Imidazole pH 8.0, 0.4M Sodium Phosphate, 1.6M Potassium Phosphate, 0.2M Nacl, Vapor diffusion, Sitting drop, temperature 298K