3LYO

CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN 95% ACETONITRILE-WATER

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	285
Detector technology	IMAGE PLATE
Collection date	1995-12
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	78.780, 78.780, 36.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.930
R-factor	0.179
Rwork	0.179
R-free	0.20800
Structure solution method	DIRECT REFINEMENT
Starting model (for MR)	6lyt
RMSD bond length	0.010
RMSD bond angle	23.950 *
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	80.000
High resolution limit [Å]	1.930
Rmerge	0.067
Total number of observations	48838 *
Number of reflections	8343
<I/σ(I)>	12.8
Completeness [%]	89.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.1 *	24-25 *	PROTEIN WAS CRYSTALLIZED FROM SODIUM ACETATE BUFFER (PH 4.3) CONTAINING 6.0% (W/V) SODIUM CHLORIDE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	drop	HAc-NaAc	10 (mM)
3	1	reservoir	HAc-NaAc	0.2 (M)
4	1	reservoir		6.0 (%(w/v))