3LYH

Crystal structure of Putative cobalamin (vitamin B12) biosynthesis CbiX protein (YP_958415.1) from Marinobacter aquaeolei VT8 at 1.60 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-23
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97915,0.97903,0.91162
Spacegroup name	P 1
Unit cell lengths	37.836, 38.615, 39.845
Unit cell angles	70.35, 83.05, 72.09

Refinement procedure

Resolution	29.618 - 1.600
R-factor	0.172
Rwork	0.170
R-free	0.20900
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.645
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.618
High resolution limit [Å]	1.600
Number of reflections	25860
Completeness [%]	96.7
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	20.0000% PEG-6000, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K