3LYH
Crystal structure of Putative cobalamin (vitamin B12) biosynthesis CbiX protein (YP_958415.1) from Marinobacter aquaeolei VT8 at 1.60 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-23 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97915,0.97903,0.91162 |
Spacegroup name | P 1 |
Unit cell lengths | 37.836, 38.615, 39.845 |
Unit cell angles | 70.35, 83.05, 72.09 |
Refinement procedure
Resolution | 29.618 - 1.600 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.20900 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.645 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.618 |
High resolution limit [Å] | 1.600 |
Number of reflections | 25860 |
Completeness [%] | 96.7 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 20.0000% PEG-6000, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |