3LYG
Crystal structure of NTF2-like protein of unknown function (YP_270605.1) from Colwellia psychrerythraea 34H at 1.61 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-03 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97925,0.97901 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.387, 57.387, 67.339 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.335 - 1.610 |
R-factor | 0.152 |
Rwork | 0.151 |
R-free | 0.18100 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.528 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 67.335 |
High resolution limit [Å] | 1.610 |
Number of reflections | 15075 |
Completeness [%] | 100.0 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 3.2000M (NH4)2SO4, 0.1M Bicine pH 9.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |