3LYG

Crystal structure of NTF2-like protein of unknown function (YP_270605.1) from Colwellia psychrerythraea 34H at 1.61 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97925,0.97901
Spacegroup name	P 43 21 2
Unit cell lengths	57.387, 57.387, 67.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.335 - 1.610
R-factor	0.152
Rwork	0.151
R-free	0.18100
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.528
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	67.335
High resolution limit [Å]	1.610
Number of reflections	15075
Completeness [%]	100.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	3.2000M (NH4)2SO4, 0.1M Bicine pH 9.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K