3LY7
Crystal structure of the periplasmic domain of CadC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 83.898, 83.898, 199.090 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 72.660 - 1.800 |
R-factor | 0.192 |
Rwork | 0.191 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lya |
RMSD bond length | 0.012 |
RMSD bond angle | 1.217 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.9) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.660 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.274 | |
Number of reflections | 39362 | |
<I/σ(I)> | 9.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.3 | 15.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.5 M ammonium sulfate, 7% (v/v) 2-propanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |