3LY1
Crystal structure of Putative histidinol-phosphate aminotransferase (YP_050345.1) from Erwinia carotovora atroseptica SCRI1043 at 1.80 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-07 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.96109,0.97946,0.97936 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.625, 150.477, 100.092 |
Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
Resolution | 29.564 - 1.800 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.20600 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.533 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.564 | 29.564 | 1.850 |
High resolution limit [Å] | 1.800 | 3.850 | 1.790 |
Rmerge | 0.059 | 0.022 | 0.675 |
Number of reflections | 158547 | 31348 | 24110 |
<I/σ(I)> | 8.36 | 24.7 | 1.4 |
Completeness [%] | 96.5 | 97 | 79.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 20.0000% iso-Propanol, 20.0000% PEG-4000, 0.1M Citrate pH 5.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |