3LXX

Crystal structure of human GTPase IMAP family member 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98013
Spacegroup name	C 2 2 21
Unit cell lengths	41.266, 102.663, 100.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.088 - 2.150
R-factor	0.254
Rwork	0.252
R-free	0.29100
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.262
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.190
High resolution limit [Å]	2.150	5.790	2.150
Rmerge	0.067	0.041	0.356
Number of reflections	11706
<I/σ(I)>	16.5
Completeness [%]	96.6	97.3	73
Redundancy	6.2	6.3	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.8	291	25% PEG-3350, 0.25M sodium/potassium tartrate, 0.1M Hepes. 1:100 dispase was added, pH 7.8, VAPOR DIFFUSION, temperature 291K
1	VAPOR DIFFUSION	7.8	291	25% PEG-3350, 0.25M sodium/potassium tartrate, 0.1M Hepes. 1:100 dispase was added, pH 7.8, VAPOR DIFFUSION, temperature 291K