3LXT
Crystal structure of Glutathione S Transferase from Pseudomonas fluorescens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9192 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.681, 57.887, 265.772 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.300 - 1.760 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.22600 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CCP4 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.070 | 0.220 |
Number of reflections | 80688 | |
<I/σ(I)> | 11.9 | 5 |
Completeness [%] | 90.2 | 54.3 |
Redundancy | 7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 278 | 0.2M MgCl2, 0.1M Tris, 25% PEG4K, Praseodymium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |