3LXR
Shigella IpgB2 in complex with human RhoA and GDP (complex C)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.580, 100.790, 50.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.530 - 1.680 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lwn |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.975 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.530 | 1.720 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.041 | 0.588 |
| Number of reflections | 45588 | 3248 |
| <I/σ(I)> | 23.76 | 2.6 |
| Completeness [%] | 98.6 | 96.9 |
| Redundancy | 4.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% (w/v) PEG 3350; post-crystallization treatment: EDTA, LiSO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
