3LXO
The crystal structure of ribonuclease A in complex with thymidine-3'-monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 294 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-14 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.592, 64.592, 65.364 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.954 - 1.549 |
| R-factor | 0.1448 |
| Rwork | 0.141 |
| R-free | 0.18130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.482 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 23146 | |
| <I/σ(I)> | 18.1 | 1.2 |
| Completeness [%] | 99.0 | 91.8 |
| Redundancy | 4.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 20% ammonium sulfate, 2M NaCl, pH 5.5., VAPOR DIFFUSION, HANGING DROP, temperature 294K |
