3LX8
Crystal structure of GDP-bound NFeoB from S. thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-12-18 |
Detector | MAR 345 mm plate |
Wavelength(s) | 1.5412 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 42.800, 42.800, 282.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.900 |
R-factor | 0.245 |
Rwork | 0.239 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lx5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.494 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.133 | 0.414 |
Number of reflections | 6494 | |
<I/σ(I)> | 11.2 | 2 |
Completeness [%] | 97.9 | 71.1 |
Redundancy | 6.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2M NaCl, 0.1M bis-Tris pH 5.5, 25% (w/v) PEG 3350, vapor diffusion, hanging drop, temperature 298K |