3LX7
Crystal structure of a Novel Tudor domain-containing protein SGF29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.802, 51.290, 44.842 |
| Unit cell angles | 90.00, 117.48, 90.00 |
Refinement procedure
| Resolution | 25.090 - 1.780 |
| R-factor | 0.20848 |
| Rwork | 0.206 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3me9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.394 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 4.800 | 1.770 |
| Rmerge | 0.071 | 0.049 | 0.301 |
| Rmeas | 0.071 | ||
| Number of reflections | 13275 | ||
| <I/σ(I)> | 12.5 | ||
| Completeness [%] | 91.1 | 91.8 | 59.5 |
| Redundancy | 3.3 | 3.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 0.1M Sodium citrate tribasic dihydrate pH 5.6, 0.2M potassium sodium tartrate, 2M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
