3LX5
Crystal structure of mGMPPNP-bound NFeoB from S. thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-24 |
| Detector | MAR 345 mm plate |
| Wavelength(s) | 1.5412 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.100, 82.200, 148.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.610 - 1.900 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hyr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.479 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.118 | 0.336 |
| Number of reflections | 23847 | |
| <I/σ(I)> | 14.2 | 3.5 |
| Completeness [%] | 97.8 | 85.4 |
| Redundancy | 5.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.02M Malic acid, 0.04M MES, 0.04M Tris, 25% (w/v) PEG 1500, pH 8.0, vapor diffusion, hanging drop, temperature 298K |
