3LWU
Crystal structure of Putative succinylglutamate desuccinylase/aspartoacylase (YP_749235.1) from Shewanella frigidimarinA NCIMB 400 at 2.10 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-07 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 126.549, 128.972, 129.574 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.623 - 2.100 |
R-factor | 0.162 |
Rwork | 0.160 |
R-free | 0.19000 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.536 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.623 | 29.623 | 2.170 |
High resolution limit [Å] | 2.100 | 4.510 | 2.100 |
Rmerge | 0.106 | 0.044 | 0.820 |
Number of reflections | 30948 | 6017 | 5539 |
<I/σ(I)> | 9.47 | 25.8 | 1.7 |
Completeness [%] | 99.7 | 99.7 | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 10.0000% Glycerol, 12.5000% polyethylene glycol 300, 0.2000M ammonium sulfate, 0.1M phosphate-citrate pH 4.5, 0.006 M zinc chloride, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |