3LWS
Crystal structure of Putative aromatic amino acid beta-eliminating lyase/threonine aldolase. (YP_001813866.1) from Exiguobacterium sp. 255-15 at 2.00 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-06 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 31 |
Unit cell lengths | 142.150, 142.150, 102.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.530 - 2.000 |
R-factor | 0.146 |
Rwork | 0.144 |
R-free | 0.19000 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.543 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.530 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.158 | 0.950 |
Number of reflections | 156755 | |
<I/σ(I)> | 9.92 | 2.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 277 | 0.2000M Mg(oAc)2, 20.0000% PEG-8000, 0.1M Cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |