3LWK
Crystal structure of human Beta-crystallin A4 (CRYBA4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9796 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 94.790, 94.790, 90.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.420 - 1.700 |
R-factor | 0.1622 |
Rwork | 0.161 |
R-free | 0.18823 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oki |
RMSD bond length | 0.016 |
RMSD bond angle | 1.551 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.030 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.104 | 0.720 |
Number of reflections | 22910 | |
<I/σ(I)> | 13.6 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293.15 | 1.5M Ammonium Phosphate, 5% Glycerol, 0.1M Tris, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |