3LWC

Crystal structure of Structural Genomics, unknown function (YP_766765.1) from Rhizobium leguminosarum BV. viciae 3841 at 1.40 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97944,0.97932
Spacegroup name	P 65 2 2
Unit cell lengths	90.663, 90.663, 45.129
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.676 - 1.400
R-factor	0.181
Rwork	0.181
R-free	0.18900
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.687
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.676	29.676	1.450
High resolution limit [Å]	1.400	3.010	1.400
Rmerge	0.083	0.011	0.011
Number of reflections	21970	4130	3934
<I/σ(I)>	16.37	42.4	2.4
Completeness [%]	99.5	99.7	95.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	2.0000M ammonium sulfate, 0.1M sodium acetate pH 4.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K