3LW7

The Crystal Structure of an Adenylate kinase-related protein bound to AMP from sulfolobus solfataricus to 2.3A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-01
Detector	ADSC QUANTUM 315
Spacegroup name	P 43 21 2
Unit cell lengths	65.876, 65.876, 206.239
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.600 - 2.300
R-factor	0.208
Rwork	0.206
R-free	0.24700
RMSD bond length	0.011
RMSD bond angle	1.367
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.109	0.737
Number of reflections	21055
<I/σ(I)>	7.9
Completeness [%]	99.0	99.9
Redundancy	6.7	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.2	277	10% PEG 3000, 0.1M Phosphate-citrate, pH 4.2, 0.2M Sodium chloride, vapor diffusion, temperature 277K