3LW6
Crystal Structure of Drosophila beta1,4-galactosyltransferase-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.835, 81.835, 133.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.600 - 1.810 |
| R-factor | 0.19029 |
| Rwork | 0.189 |
| R-free | 0.22059 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Brominated structure was solved using SAD at 2.0 A resolution and the cordinated are used as the stating model. |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.991 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Number of reflections | 41321 | |
| <I/σ(I)> | 35 | 4.2 |
| Completeness [%] | 98.0 | 85 |
| Redundancy | 11.9 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 10-20 mg/ml protein with 33 mm UDP and 66 mM MnCl2 with a precipitation solution containing 100 mM Tris.HCl, 1 M NaCl, 15% MPD and 5% PEG 4K., pH 8.0, VAPOR DIFFUSION, temperature 298K |
