3LVY
Crystal Structure of Carboxymuconolactone Decarboxylase Family Protein SMU.961 from Streptococcus mutans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-03 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97921 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 97.996, 73.741, 183.708 |
Unit cell angles | 90.00, 98.68, 90.00 |
Refinement procedure
Resolution | 34.160 - 2.100 |
R-factor | 0.187 |
Rwork | 0.184 |
R-free | 0.23600 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.238 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.160 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 75930 | |
<I/σ(I)> | 12.6 | 2 |
Completeness [%] | 99.5 | 98.5 |
Redundancy | 3.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M Hepes pH 7.5, 0.2 M Ammonium Sulphate, 25 % PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |