3LVX
Crystal structure of human alpha-defensin 1 (I6A mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-05 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 118.069, 118.069, 118.069 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.630 |
| R-factor | 0.16868 |
| Rwork | 0.168 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gny |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.343 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.167 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.161 | 0.790 |
| Number of reflections | 9272 | |
| <I/σ(I)> | 17.4 | 2 |
| Completeness [%] | 99.0 | 96.6 |
| Redundancy | 10.9 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2% PEG 400; 0.1 M HEPES-Na, pH 7.5; 2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
