3LVV
BSO-inhibited ScGCL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 117.863, 117.863, 165.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.350 - 2.200 |
| R-factor | 0.18302 |
| Rwork | 0.181 |
| R-free | 0.23851 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ig5 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.883 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.057 | 0.503 |
| Number of reflections | 59891 | |
| <I/σ(I)> | 25.4 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 291 | 100 mM Hepes, pH 6.8, 12% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
