3LVU
Crystal structure of ABC transporter, periplasmic substrate-binding protein SPO2066 from Silicibacter pomeroyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.384, 75.517, 78.477 |
| Unit cell angles | 82.63, 80.29, 77.42 |
Refinement procedure
| Resolution | 50.000 - 1.790 |
| R-factor | 0.17084 |
| Rwork | 0.169 |
| R-free | 0.20476 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.468 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.116 | 0.802 |
| Number of reflections | 94748 | |
| <I/σ(I)> | 23 | 2.37 |
| Completeness [%] | 97.5 | 96.3 |
| Redundancy | 4.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.2M Sodium iodide, 20% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
