3LVT
The Crystal Structure of a Protein in the Glycosyl Hydrolase Family 38 from Enterococcus faecalis to 2.55A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-07 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 132.728, 134.947, 106.329 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.040 - 2.550 |
R-factor | 0.225 |
Rwork | 0.222 |
R-free | 0.28200 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.387 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.082 | 0.635 |
Number of reflections | 31505 | |
<I/σ(I)> | 10.5 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 277 | 0.4M NaH2PO4/1.6M K2HPO4, 0.1M Imidazole pH 8.0, 0.2M Sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K |