3LVR
The crystal structure of ASAP3 in complex with Arf6 in transition state soaked with Calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.3838 |
| Spacegroup name | P 62 |
| Unit cell lengths | 146.450, 146.450, 49.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.460 - 3.380 |
| R-factor | 0.21095 |
| Rwork | 0.207 |
| R-free | 0.28129 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lvq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.058 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.460 | 3.500 |
| High resolution limit [Å] | 3.380 | 3.380 |
| Rmerge | 0.101 | 0.575 |
| Number of reflections | 8310 | |
| <I/σ(I)> | 19 | 3.5 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 5.85 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 13% PEG 8000, 150mM Magnesium Acetate, 100mM MOPS(pH 7.5); Crystals were then soaked in 30mM CaCl2 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
