3LVG
Crystal structure of a clathrin heavy chain and clathrin light chain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Collection date | 2002-03-03 |
| Wavelength(s) | 1.017 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 228.560, 228.560, 710.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 7.940 |
| Rwork | 0.419 |
| R-free | 0.42500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.853 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 250.000 | 8.550 |
| High resolution limit [Å] | 7.900 | 7.900 |
| Number of reflections | 10700 | |
| <I/σ(I)> | 20 | 2.8 |
| Redundancy | 9 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 200mM citrate, 16-22% glycerol, 2% trifluoroethanol, vapor diffusion, hanging drop, temperature 298K |
