3LVF
Crystal Structure of holo Glyceraldehyde-3-phosphate dehydrogenase 1 (GAPDH1) from methicillin resistant Staphylococcus aureus MRSA252 at 1.7 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-05-14 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.180, 104.874, 91.191 |
Unit cell angles | 90.00, 107.72, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hdg |
RMSD bond length | 0.006 |
RMSD bond angle | 1.040 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.8L) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0095) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.780 | 30.780 | 1.760 |
High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
Rmerge | 0.059 | 0.035 | 0.430 |
Rmeas | 0.059 | ||
Total number of observations | 448834 | 50949 | 42059 |
Number of reflections | 126584 | ||
<I/σ(I)> | 8.7 | 25.2 | 2.4 |
Completeness [%] | 94.5 | 99 | 93.6 |
Redundancy | 3.52 | 3.64 | 3.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Tris-HCl, pH8.5, 20% (w/v)PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |