3LV0
Crystal structure of extragenic suppressor protein suhB from Bartonella henselae, native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-13 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.060, 138.600, 67.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.19257 |
| Rwork | 0.191 |
| R-free | 0.21816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3luz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.434 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.610 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.061 | 0.552 |
| Number of reflections | 66080 | |
| <I/σ(I)> | 19.58 | 3.5 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 7.9 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 24 mg/mL protein against 0.2M MgCl2, 0.1 M Tris pH 8.5, 30% PEG 400, crystal tracking ID 205526c1, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
