3LUP

Crystal structure of fatty acid binding DegV family protein SAG1342 from Streptococcus agalactiae

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97931
Spacegroup name	P 21 21 21
Unit cell lengths	39.321, 84.205, 87.541
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.650
R-factor	0.20019
Rwork	0.197
R-free	0.26525
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.303
Data reduction software	HKL-2000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.127	0.643
Number of reflections	8910
<I/σ(I)>	25.3	4.39
Completeness [%]	100.0	100
Redundancy	6.9	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	297	0.16 M MgCl2, 80 mM Tris-Cl, 24 % PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K
1	VAPOR DIFFUSION, SITTING DROP	8.5	297	0.16 M MgCl2, 80 mM Tris-Cl, 24 % PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K