3LUF
Structure of probable two-component system response regulator/GGDEF domain protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 |
Unit cell lengths | 41.195, 53.699, 57.887 |
Unit cell angles | 75.39, 80.99, 77.41 |
Refinement procedure
Resolution | 40.000 - 1.760 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22600 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.498 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.790 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.063 | 0.216 |
Number of reflections | 44744 | |
<I/σ(I)> | 56.3 | 6.9 |
Completeness [%] | 97.4 | 95.4 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1 M Bis-Tris pH 5.5, 25% PEG 3350, 0.2M MgCl2, Vapor diffusion, Sitting drop, temperature 298K |