3LUA
Crystal structure of a Signal receiver domain of Two component Signal Transduction (Histidine Kinase) from Clostridium thermocellum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 94.100, 94.100, 68.009 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.050 - 2.400 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.26700 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
<I/σ(I)> | 6.2 | 5.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.9 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.2M Sodium acetate pH 7.0, 20% PEG 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 292K |