3LTU
5-SeMe-dU containing DNA 8mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2009-10-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 43.078, 43.078, 23.814 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.460 - 1.400 |
| R-factor | 0.17584 |
| Rwork | 0.175 |
| R-free | 0.20041 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dns |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.108 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.087 | 0.136 |
| Number of reflections | 4661 | |
| <I/σ(I)> | 18.9 | 14.5 |
| Completeness [%] | 97.8 | 99.3 |
| Redundancy | 23 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 10% v / v MPD, 40 mM Na Cacodylate pH 6.0, 12 mM Spermine tetra-HCl, 80 mM Sodium Chloride, 20 mM Magnesium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
