3LTJ
Structure of a new family of artificial alpha helicoidal repeat proteins (alpha-Rep) based on thermostable HEAT-like repeats
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9197 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 64.630, 85.180, 34.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.744 - 1.800 |
R-factor | 0.1768 |
Rwork | 0.174 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ltm |
RMSD bond length | 0.005 |
RMSD bond angle | 0.892 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.064 | 0.426 |
Number of reflections | 17826 | |
<I/σ(I)> | 17 | 2.9 |
Completeness [%] | 97.8 | 97.6 |
Redundancy | 5.4 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 4% MPD, 0.1M Na citrate pH 5.6, 0.1M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 292K |