3LSS
Trypanosoma brucei seryl-tRNA synthetase in complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-19 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | H 3 2 |
Unit cell lengths | 175.652, 175.652, 248.518 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.570 - 1.950 |
R-factor | 0.169 |
Rwork | 0.168 |
R-free | 0.19500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3lsq |
RMSD bond length | 0.013 |
RMSD bond angle | 1.331 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.090 | 0.808 |
Number of reflections | 106754 | |
<I/σ(I)> | 9.1 | 1.5 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 8 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop vapor diffusion | 7 | 298 | 1 ul 11 mg/ml protein in SGPP buffer mixed with 0.5 ul 2.4 M sodium malonate; crystal then soaked in 3.4 M sodium malonate (pH 7.0) supplemented with 10 mM ATP for 25 minutes before freezing., sitting drop vapor diffusion, temperature 298K |