3LSR
Crystal structure of DesT in complex with duplex DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | I 41 |
Unit cell lengths | 79.358, 79.358, 145.458 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.380 - 2.550 |
R-factor | 0.22926 |
Rwork | 0.228 |
R-free | 0.26477 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lsp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.423 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.049 |
Number of reflections | 14862 |
<I/σ(I)> | 40.4 |
Completeness [%] | 96.1 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | grown under mineral oil | 7 | 291 | ML- 0.1M Mes pH7.0, 9% PEG 20K. Protein sample- 0.3 mM protein, 0.3 mM oligo, 0.3 mM 18:1delta9-CoA. Drop- 2ul ML + 2 ul protein + 0.5 uL 1M ammonium sulfate., grown under mineral oil, temperature 291K |