3LSP
Crystal Structure of DesT bound to desCB promoter and oleoyl-CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | I 41 |
Unit cell lengths | 78.385, 78.385, 147.988 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.730 - 2.660 |
R-factor | 0.22515 |
Rwork | 0.223 |
R-free | 0.26136 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.283 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.650 |
Rmerge | 0.072 |
Number of reflections | 12753 |
<I/σ(I)> | 28.4 |
Completeness [%] | 98.9 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | ML- 0.1 M Mes pH7.0, 9%PEG 20K. Protein sample- 0.3 mM protein, 0.3 mM oligo, 0.3 mM 18:1delta9-CoA. Drop- 2ul ML + 2ul protein + 0.5 ul 1M ammonium sulfate., VAPOR DIFFUSION, SITTING DROP, temperature 291K |