3LSO
Crystal structure of Putative membrane anchored protein from Corynebacterium diphtheriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.205, 95.517, 160.778 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.750 |
| R-factor | 0.2165 |
| Rwork | 0.215 |
| R-free | 0.25107 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.479 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.096 | 0.780 |
| Number of reflections | 30141 | |
| <I/σ(I)> | 27.4 | 3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 297 | 20% PEG 8000, 0.1M citrate-phosphate pH 4.2, 0.2M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
