3LSK
Pyranose 2-oxidase T169S acetate complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-13 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 100.256, 102.454, 136.716 |
Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.950 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.25600 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.023 |
RMSD bond angle | 2.004 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | FFT |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.700 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 198647 | |
<I/σ(I)> | 6.2 | 1.8 |
Completeness [%] | 98.8 | 99.3 |
Redundancy | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 298 | 0.1M sodium acetate, 0.05M MgCl2, 6% (w/v)% monomethylether PEG 2000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |