3LSH
Pyranose 2-oxidase T169A, monoclinic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-19 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.403, 102.731, 137.817 |
| Unit cell angles | 90.00, 91.02, 90.00 |
Refinement procedure
| Resolution | 28.500 - 1.900 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.24600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.968 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.500 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 217370 | |
| <I/σ(I)> | 12.7 | 2.7 |
| Completeness [%] | 98.8 | 98.4 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 298 | 0.1M MES, 50MM MgCl2, 10% (W/V)% monomethylether PEG 2000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
