3LSD
N-Domain of human adhesion/growth-regulatory galectin-9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 37.813, 37.813, 153.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.030 - 2.030 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a3k |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.817 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.030 | 2.120 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.074 | 0.215 |
| Number of reflections | 8444 | |
| <I/σ(I)> | 17.8 | 6.7 |
| Completeness [%] | 95.6 | 77.5 |
| Redundancy | 6.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | 26 % (w/v) PEG 4000, 0.25 M Na acetate, 0.1 M Tris (pH 8.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
