3LRY
Crystal structure of synthetic HIV-1 capsid C-terminal domain (CCA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-02-25 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 82.907, 58.932, 58.732 |
Unit cell angles | 90.00, 133.45, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.980 |
R-factor | 0.216 |
Rwork | 0.213 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a43 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.593 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.636 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.065 | 0.578 |
Number of reflections | 13819 | |
<I/σ(I)> | 14.5 | 2 |
Completeness [%] | 96.0 | 95.6 |
Redundancy | 3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M HEPES, 0.8 M potassium phosphate monohydrate, 0.8M potassium phosphate monobasic, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |