3LRV

The Prp19 WD40 Domain Contains a Conserved Protein Interaction Region Essential for its Function.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	200
Detector technology	CCD
Detector	MAR CCD 165 mm
Spacegroup name	P 42 21 2
Unit cell lengths	83.110, 83.110, 126.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.160 - 2.600
R-factor	0.222
Rwork	0.220
R-free	0.27000
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.397
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.080	0.445
Number of reflections	14158
<I/σ(I)>	16	3.5
Completeness [%]	99.2	99.6
Redundancy	6.3	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	291	2.0 M ammonium sulfate and 0.1 M Tris at pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1		7.5	291	2.0 M ammonium sulfate and 0.1 M Tris at pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1		7.5	291	2.0 M ammonium sulfate and 0.1 M Tris at pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K