3LRS
Structure of PG16, an antibody with broad and potent neutralization of HIV-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.317, 165.085, 81.363 |
| Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
| Resolution | 30.590 - 2.370 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nlo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.154 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.410 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.126 | 0.512 |
| Number of reflections | 71375 | |
| <I/σ(I)> | 15.73 | 1.49 |
| Completeness [%] | 81.5 | 25.4 |
| Redundancy | 3.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10.5 | 293 | 0.74M Potassium Dihydrogen Phosphate, 1.10M Sodium Dihydrogen Phosphate, 0.2M Lithium Sulfate Monohydrate, 0.1 M CAPS, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
