3LR8
Self-assembly of spider silk proteins is controlled by a pH-sensitive relay
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.193, 91.193, 143.546 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.100 - 2.300 |
| R-factor | 0.19436 |
| Rwork | 0.192 |
| R-free | 0.24654 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NT wild-type |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.487 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.060 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.144 | 0.341 |
| Number of reflections | 15139 | |
| <I/σ(I)> | 2 | 7.8 |
| Redundancy | 11.4 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | Ammonium sulphate, PEG 400, VAPOR DIFFUSION | ||
| 1 | VAPOR DIFFUSION | Ammonium sulphate, PEG 400, VAPOR DIFFUSION |
