3LR7
Ferric horse heart myoglobin, nitrite adduct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.266, 28.532, 62.932 |
| Unit cell angles | 90.00, 105.43, 90.00 |
Refinement procedure
| Resolution | 21.850 - 1.600 |
| R-factor | 0.1957 |
| Rwork | 0.193 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1dwr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.993 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.850 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.056 | 0.298 |
| Number of reflections | 15927 | |
| <I/σ(I)> | 9.9 | 3 |
| Completeness [%] | 98.0 | 96 |
| Redundancy | 3 | 2.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | seeding | 7.4 | 295 | 2.76M ammonium sulfate, 100 mM TrisHCl, 1 mM EDTA, pH 7.4, seeding, temperature 295K |
