3LR6

Self-assembly of spider silk proteins is controlled by a pH-sensitive relay

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 32 2 1
Unit cell lengths	68.670, 68.670, 97.342
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.400 - 2.200
R-factor	0.20929
Rwork	0.207
R-free	0.24794
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.333
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.440	2.257
High resolution limit [Å]	2.200	2.200
Rmerge	0.089	0.345
Number of reflections	12619
<I/σ(I)>	7.4	2.2
Redundancy	4.5	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION			Ammonium sulphate, PEG 400, VAPOR DIFFUSION
1	VAPOR DIFFUSION			Ammonium sulphate, PEG 400, VAPOR DIFFUSION
1	VAPOR DIFFUSION			Ammonium sulphate, PEG 400, VAPOR DIFFUSION