3LR5
Periplasmic domain of the risS sensor protein from Burkholderia pseudomallei, iodide phased at neutral pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-04 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.040, 76.010, 88.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.610 - 2.300 |
R-factor | 0.206 |
Rwork | 0.203 |
R-free | 0.25200 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.504 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.610 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.092 | 0.410 |
Number of reflections | 13048 | 947 |
<I/σ(I)> | 15.79 | 4.6 |
Completeness [%] | 98.9 | 99.8 |
Redundancy | 6.69 | 6.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M BisTris Propane pH 6.5, 0.2 M NaI, 25% PEG 3350, crystal tracking ID 203088f3, affinity tag not removed prior to crystallization, VAPOR DIFFUSION, SITTING DROP, temperature 289K |