3LR4

Periplasmic domain of the risS sensor protein from Burkholderia pseudomallei, barium phased at low pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-04
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	57.780, 57.780, 75.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.910 - 1.900
R-factor	0.188
Rwork	0.186
R-free	0.22600
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.385
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.910	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.046	0.321
Number of reflections	22306	1650
<I/σ(I)>	33.77	4.6
Completeness [%]	99.7	99.6
Redundancy	9.29	4.17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	289	growth: 15% PEG 3350, 0.1 M succinate; soak and cryo-protection: 30% PEG 3350, 0.45 M BaCl2, 0.1 M Na citrate pH 4.5, crystal tracking ID 206692g7, VAPOR DIFFUSION, SITTING DROP, temperature 289K